The Effect of Room-Temperature Storage on the Stability of Compounds in DMSO

Author(s): Barbara A. Kozikowski ; Thomas M. Burt ; Debra A. Tirey ; Lisa E. Williams ; Barbara R. Kuzmak ; David T. Stanton ; Kenneth L. Morand ; Sandra L. Nelson
Source: Journal of Biomolecular Screening Volume: 8 Number: 2 Page: 205 -- 209

Abstract: The stability of approximately 7200 compounds stored as 20-mM DMSO
solutions under ambient conditions was monitored for 1 year. Compound
integrity was measured by flow injection analysis using positive and
negative electrospray ionization mass spectrometry. Each sample was assessed
at the beginning of the study, after 12 months of storage, and at a
randomized time point between the initial and final time points of the
study. The relationship between length of storage and the probability of
observing the compound was described by a repeated-measures logistic
regression model. The probability of observing the compound was 92% after 3
months of storage at room temperature, 83% after 6 months, and 52% after 1
year in DMSO. An acceptable limit for compound loss and corresponding
maximum storage time for samples in DMSO can be determined based on these
results.